Final project report

This project aimed to develop and implement a new framework for improving bioproduction under harsh conditions, resulting in economic advantages for industrial processes.Our approach addresses the failure of reactions required for bio-production, due to inhibition, denaturation, mis-folding or disorder of individual enzymes, using E. coli as a model system.We have developed and implemented theoretical and experimental frameworks for bioproduction at low intracellular pH, and updated and synthesized frameworks for bioproduction at high temperature.Our approach is complementary to efforts to improve microbial robustness by engineering the cell membrane and has advantages relative to evolutionary-based organism improvement by prioritizing bio-production. PRODUCTS 2.