Engineering B-ketoadipic Acid Production in Pseudomonas putida KT2440: Cooperative Research and Development (Final Report)

OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) 2026 Open access

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Authors: Transportation Office. Bioenergy Technologies Office USDOE Office of Energy Efficiency and Renewable Energy (EERE), Robert M. Baldwin, USDOE Office of Critical Minerals and Energy Innovation (CMEI), Gregg T. Beckham, Carol Gomez, Ramesh Jha, Taraka Dale, Young-Mo Kim, Monica Chavez, Beth A. Hofstad, Mukund Karanjikar, Øyvind Hatlevik
DOI: 10.2172/3016090
Cited by: 0

Abstract

We propose a 2-year project to enable commercialization of an Agile Biofoundry (ABF) related, performance-advantaged bioproduct, B-ketoadipic acid (BKA), in an ABF-relevant, industrially-relevant host, Pseudomonas putida KT2440. Informed by techno-economic analysis (TEA), our BKA production goals are to enable a 40 g/L titer, 0.5 g/L/h productivity, and a 40% molar yield from hydrolysate sugars at the 1 kg scale. This material will be used to produce BKA-nylons.

Fields of Study & Subjects

Microbial Metabolic Engineering and Bioproduction biodegradable polymer synthesis and properties Biochemical and biochemical processes

Publication details

Journal / source
OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information)
Publication date
29 January 2026
Language
EN