TCF Base Technology-Specific Final Report: Engineering Enzymes for Crystalline PET Substrate

OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) 2026 Open access

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Authors: Office of Technology Commercialization (OTC), Gregg T. Beckham, USDOE Office of Critical Minerals and Energy Innovation (CMEI), Michelle Reed, Jason S. DesVeaux, Brenna Norton‐Baker, Natasha Murphy
DOI: 10.2172/3018255
Cited by: 0

Abstract

The primary objective of this project was to develop a new polyethylene terephthalate (PET) hydrolase enzyme to depolymerize relevant PET substrates for Birch Biosciences, using high-throughput protein expression, purification, and assaying systems and machine learning-guided enzyme design. As a secondary project objective, we also aimed to develop a more energy-efficient ethylene glycol (EG) recovery strategy relative to distillation.

Fields of Study & Subjects

Enzyme Catalysis and Immobilization Enzyme Production and Characterization biodegradable polymer synthesis and properties

Publication details

Journal / source
OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information)
Publication date
9 February 2026
Language
EN