HPTLC - Based Quality Assessment and Standardization of Baljeevan Syrup

Background: Baljeevan Syrup is a classical polyherbal Ayurvedic formulation containing Piper longum, Asparagus racemosus, Rosa centifolia, Emblica officinalis, Withania somnifera, and Acorus calamus known for their immunomodulatory, adaptogenic, and nutritive properties. With the increasing global acceptance of herbal medicines, quality control and standardization of such formulations have become essential. Aim: To develop and validate a High-Performance Thin-Layer Chromatography (HPTLC) method for the phytochemical standardization of Baljeevan Syrup. Materials and Methods: An optimized HPTLC protocol was established for the simultaneous qualitative and quantitative estimation of six phytoconstituents, mainly piperine, gallic acid, quercetin, kaempferol, rutin, and ellagic acid. Chromatographic fingerprints, retention factor (Rf) values, and densitometric evaluation were used for analysis. Results: The developed method demonstrated high specificity, resolution, and reproducibility. Well-resolved chromatographic bands with consistent Rf values confirmed the presence of marker compounds. The validated method enabled accurate estimation of all six markers, ensuring reliability for routine analysis. Conclusion: The study establishes a reproducible and validated HPTLC protocol for the quality control of Baljeevan Syrup. This method supports batch-to-batch consistency, scientific validation, and regulatory compliance, thereby strengthening the evidence base for Ayurvedic formulations.