Turbo-RIP: A Protocol for TurboID-based RNA Immunopurification to Map RNA Landscapes in Plant Biomolecular Condensates

, validation of TurboID activity, and RNA recovery. The protocol successfully captured processing body-associated RNAs with minimal background. Turbo-RIP enables systematic mapping of RNA populations within plant condensates under diverse conditions. The protocol requires 3-5 days from sample preparation to RNA isolation, with construct validation taking 2-4 weeks. All procedures use standard laboratory equipment, making Turbo-RIP accessible for plant molecular biology laboratories. Key features • Apply TurboID-based proximity labeling specifically for RNA capture in plant condensates. • Optimize experimental conditions for different plant species and condensate types. • Implement quality control measures and data analysis pipelines.