Advancing EAE Modeling: Establishment of a Non-Pertussis Immunization Protocol for Multiple Sclerosis
Abstract
) peptide. This model recapitulates hallmark features of MS that include demyelination, neuroinflammation, motor deficits, and neuropathic pain. Importantly, it retains sex-specific differences in disease onset and pathology, providing a more physiologically and clinically relevant platform for mechanistic and translational MS research. Key features • Establishes a reproducible clinically relevant EAE protocol in C57BL/6 mice that induces MS-like neurological deficits without pertussis toxin. • Recapitulates hallmark MS pathology, including neuroinflammation, demyelination, axonal injury, and neuropathic pain. • Eliminates the off-target effects of pertussis toxin, which confound GPCR-mediated signaling, T-cell responses, and neuroimmune interactions.
Fields of Study & Subjects
Multiple Sclerosis Research Studies
Peripheral Neuropathies and Disorders
Vagus Nerve Stimulation Research
Publication details
- Journal / source
- BIO-PROTOCOL
- Publisher
- Bio-Protocol, LLC
- Volume
- 16
- Issue
- 1390
- Pages
- e5589–e5589
- Publication date
- 1 January 2026
- Language
- EN