Using Combined Fluorescent In Situ Hybridization With Immunohistochemistry to Co-localize mRNA in Diverse Neuronal Cell Types
Abstract
technology with standard immunohistochemistry techniques. • Visual simultaneous detection of mRNA and protein expression within murine brain tissue to identify mRNA transcripts within specific neuronal subpopulations. • Using a custom macro code for FIJI/ImageJ for an efficient analysis of total mRNA expression or mRNA co-localization with neuronal subtypes. • This protocol can be used to detect any mRNA and protein combination given appropriate antigen retrieval and tissue preparation within any tissue type of interest.
Fields of Study & Subjects
RNA Research and Splicing
Developmental Biology and Gene Regulation
Neurogenesis and neuroplasticity mechanisms
Publication details
- Journal / source
- BIO-PROTOCOL
- Volume
- 16
- Issue
- 1390
- Pages
- e5591–e5591
- Publication date
- 1 January 2026
- Language
- EN