Quick and Cheap: Optimized Purification and Concentration of Bacteriophages Produced in Rich Culture Media
Abstract
, is a lactic acid bacterium that lives in harsh oenological ecosystems and grows only in rich and complex media such as Man-Rogosa-Sharpe (MRS) or fruit juice-based media in laboratory conditions. This raises experimental challenges in pure and concentrated phage preparations for further uses such as structure-function studies. Key features • Simple, rapid, and cheap, this method provides a fast and easy approach for efficient bacteriophage purification and concentration. • The method meets the structural study requirements of phage particles. • The method is compatible with bacteria that grow only in complex and rich culture media. • This protocol is also compatible with the purification of phages that produce low-titer lysates.
Fields of Study & Subjects
Bacteriophages and microbial interactions
Bacterial Genetics and Biotechnology
Advanced Electron Microscopy Techniques and Applications
Publication details
- Journal / source
- BIO-PROTOCOL
- Volume
- 16
- Issue
- 1391
- Pages
- e5608–e5608
- Publication date
- 1 January 2026
- Language
- EN