Simultaneous Immunofluorescence-Based In Situ mRNA Expression and Protein Detection in Bone Marrow Biopsy Samples

BIO-PROTOCOL 2026 Open access

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Authors: Alba Lillo Sierras, Sandro Bräunig, Hongzhe Li, Stefan Scheding
DOI: 10.21769/bioprotoc.5612
Cited by: 0

Abstract

) cells in the bone marrow of patients with acute lymphoblastic leukemia (ALL). Key features • The protocol describes the simultaneous visualization of RNA target transcripts and protein expression. • In situ RNA and surface marker analysis was established for routine human bone marrow biopsies.

Fields of Study & Subjects

Acute Lymphoblastic Leukemia research Molecular Biology Techniques and Applications Single-cell and spatial transcriptomics

Publication details

Journal / source
BIO-PROTOCOL
Volume
16
Issue
1396
Pages
e5612–e5612
Publication date
1 January 2026
Language
EN