Purification of the Active-State G Protein-Coupled Receptor ADGRL4 for Cryo-Electron Microscopy Using a Modular Tag System and a Tethered mini-Gq

BIO-PROTOCOL 2026 Open access

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Authors: David Mordechai Favara, Christopher G. Tate
DOI: 10.21769/bioprotoc.5617
Cited by: 1

Abstract

increases stability and receptor cell-surface expression. • Modular purification tagging configuration provides freedom to change purification methodologies without having to perform additional receptor engineering or cloning.

Fields of Study & Subjects

Advanced Electron Microscopy Techniques and Applications Electron Spin Resonance Studies Force Microscopy Techniques and Applications

Publication details

Journal / source
BIO-PROTOCOL
Publisher
Bio-Protocol, LLC
Volume
16
Issue
1392
Pages
e5617–e5617
Publication date
1 January 2026
Language
EN