A Dual-gRNA CRISPR/Cas9 System for Efficient Generation of Large Fragment Deletions in Poplar

BIO-PROTOCOL 2026 Open access

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Authors: Guoqian Yang, Yu Yang, Vijaya Vulavala, Nidhi Dwivedi, Chang-Jun Liu
DOI: 10.21769/bioprotoc.5734
Cited by: 1
References: 24

Abstract

-mediated transformation and tissue culture-based regeneration. • Simplifies mutant screening through PCR-based genotyping, minimizing reliance on sequencing. • Facilitates simultaneous editing of homologous genes in bioenergy-relevant poplar lines.

Fields of Study & Subjects

CRISPR and Genetic Engineering Chromosomal and Genetic Variations Pluripotent Stem Cells Research

Publication details

Journal / source
BIO-PROTOCOL
Publisher
Bio-Protocol, LLC
Volume
16
Issue
1400
Pages
e5734–e5734
Publication date
1 January 2026
Language
EN