Synergistic effects of oxypeucedanin and temozolomide on viability, proliferation, apoptosis, and migration of T98G malignant glioblastoma cells.

Objective: Glioblastoma multiforme (GBM), an aggressive primary brain tumor, distinguished by an invasive growth pattern and resistance to current therapeutic strategy. This study investigates the potential of Oxypeucedanin (OP) as a natural compound to induce apoptosis and inhibit proliferation in T98G GBM cells, either alone or in combination with Temozolomide (TMZ). Materials and Methods: T98G cells were exposed to OP and TMZ individually and in combination. Then, cell viability (MTT assay), cell proliferation (using trypan blue), mRNA expression (qRT-PCR), Cell cycle and apoptosis (flow cytometry), and migration (wound healing assay) were evaluated. Results: levels, suggesting enhanced apoptotic susceptibility and lowered proliferation capacity. Furthermore, the wound healing assay confirmed reduced migration in T98G cells, particularly in the combination treatment group. Conclusion: This study suggests the potential of OP as a complementary therapeutic agent alongside TMZ for GBM treatment.