The Correlation between miR-let-7b Expression, Sperm Parameters, Oxidative Stress Markers, and Hormonal Levels in Oligoasthenoteratozoospermia Men.

Background: microRNAs (miRNAs) are small, endogenous, single-stranded RNA molecules that regulate gene expression and play a role in various disease states. The let-7 family of miRNAs constitutes an important class of regulators for diverse cellular functions ranging from cell proliferation, differentiation in spermatogenesis. The aim of study review the current state of correlation between miR-let-7b expression and sperm parameters, oxidative stress markers, and hormonal levels in Oligoasthenotratozoospermia (OAT) men. Materials and Methods: was evaluated by real time-quantitative polymerase chain reaction (RT-qPCR). The DNA fragmentation index (DFI) was measured using TUNEL assay technique. Using World Health Organization (WHO, 2010) criteria, sperm parameters were evaluated. Oxidative stress markers, i.e., total antioxidant capacity (TAC), reactive oxidative stress (ROS), and malondialdehyde (MDA), as well as hormonal profile [luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone] were determined by ELISA kit. Results: level, but positively correlated with the sperm DNA fragmentation (r=+0.691, P=0.011), ROS (r=0.376, P=0.031), and MDA (r=0.201, P=0.033). Conclusion: level in OAT patients may be associated with the susceptibility and progression of infertility.