Effectiveness Of Black Soldier Fly Larvae Oil Ointment On The Healing Process Of Incision Wounds In Mice

Black soldier fly (BSF) larvae contain proteins and fatty acids that have significant potential in enhancing the wound healing process. However, the application of BSF larvae oil in the form of a wound ointment has not been extensively explored. Therefore, this study aimed to determine the effect of BSF larvae oil ointment application on the wound healing process (wound morphology, skin histology, and modulation of leukocyte components) in an incision wound model. This experimental study used a completely randomized design with Balb/c mice as test subjects. The test subjects were divided into six different groups: no treatment (NT), gentamicin ointment (GTC), ointment base (OB), and BSF larvae oil ointment 5% (BSFO 5%), BSF larvae oil ointment 10% (BSFO 10%), and BSF larvae oil ointment 15% (BSFO 15%). The ointment was applied twice a day at 0.15 g per application, and wound healing parameters were observed over 14 days. The results showed that BSFO 5% significantly improved wound morphology, reduced wound length, and enhanced histological components of the wound compared to the control and all other treatments. Additionally, BSFO 5% was able to normalize leukocyte levels after 14 days. Furthermore, BSFO 15% was proven effective in modulating lymphocyte levels compared to other groups. These findings emphasize the potential use of BSF larvae oil ointment in enhancing wound healing and regulating immune responses by increasing lymphocyte levels, and open new opportunities for future technology development in wound therapy and care.