ANTI-INFLAMMATORY POTENTIAL EVALUATION AND MORPHOLOGICAL CHARACTERISATION OF NANOHYDROXYAPATITE INCORPORATED GREEN SYNTHESIZED NANOCOMPOSITE- AN IN-VITRO STUDY

Objective: This research intended to investigate the anti-inflammatory capabilities of nano-hydroxyapatite (nHAP) based nanocomposites developed through green synthesis from citrus fruit peels. Methods: The experimental design involved the green synthesis of nHAP-mediated chitosan-based nanocomposites from Citrus reticulata and Citrus limonum peel extract. The anti-inflammatory potential was assessed using the Bovine serum albumin (BSA) denaturation assay, membrane stabilization assay and Egg albumin denaturation assays. The developed nanocomposite was also subjected to characterization using X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy analysis. Results: The successful generation of the nanocomposite based on nHAP was carried out. The generated nanocomposite exhibited anti-inflammatory behavior. The characterization using XRD and FTIR analysis also confirmed the presence of nHAP particles from the green precursors. Conclusion: The C. reticulata and C. limonum peel extract mediated nHAP-based nanocomposite exhibited potential anti-inflammatory effects its suggested use in biomedical applications.