OPTIMISATION AND CHARACTERIZATION OF TERBINAFINE-LOADED TOPICAL SOLID LIPID NANOPARTICLES

Objective: The present study aimed to prepare a nanoformulation using GMS and/orPrecirol ATO5and Tween 80 (1% and 2%) to develop and optimize solid lipid nanoparticles with the potential to enhance oral bioavailability. Methods: The factorial design was selected to study the impact of two solid lipid formulation parameters on the particle size (PS), zeta potential (PS), entrapment efficiency (EE), and in-vitro drug release. The emulsification technique was used to develop solid lipid nanoparticles, and the prepared nanoparticles were evaluated using design of expert (DoE) software. Results: The drug estimation was found linear between 5and 40µg/mL, and the melting point was found to be 195 oC. Enhancing the lipid content (GMS or Precirol ATO5) resulted in an increase in PS. However, increasing the concentration of the surfactant (Tween 80) reduced the PS.Among the two lipids used as a qualitative factor, the desirability function was more with PrecirolATO5 formulations. The numerical optimization suggested an optimized formulation containing 2% Tween 80 and 5.5% Precirol ATO5. Among the experimental batches, formulation F6 (2% Tween 80 and 3% Precirol ATO5) exhibited a PS of 98.3±5.12nm, an EE of 81.5±6.27%, and a drug release of 77.5±8.09% over 10 h. Conclusion: The TB-loaded SLN were successfully formulated by the emulsification method. Among the two lipids used as a qualitative factor, the desirability function was more with PrecirolATO5 formulations. This may potentially enhance oral bioavailability, as suggested by nanoscale PS and improved in vitro drug release.