Phytochemical Profile, TLC Profiling and Antioxidant Potential of Prunus Avium

This work looks at the Chromatographic content of the hydro-alcoholic extract of Prunus avium leaf and its antioxidant activity. The presence of essential metabolites was confirmed by preliminary phytochemical screening for glycosides, flavonoids, alkaloids, steroids, volatile oils, and tannins, indicating the plant's ethnomedicinal value. To maximise the separation and visibility of constituent substances, TLC was done using three solvent systems of varied polarity. Under 254 nm and 365 nm UV light, distinct, well-resolved bands were observed, indicating a different range of polar and mid-polar phytochemicals. Further antioxidant activity was assessed using DPPH and ABTS radical scavenging assays. The extensive chromatographic and phytochemical profiles obtained in the study highlight the potential of P. avium as a valid phytotherapeutic candidate, setting the stage for future bioactive-guided reclusion and anticancer testing. Keywords: Prunus avium, sweet cherry, Chromatographic content, TLC analysis, hydroalcoholic extract.