Investigation of phytochemical and pharmacological activities of the ethanolic extract of Nyctanthes arbor-tristis leaves in swiss albino mice

Background: In Bangladesh, Nyctanthes arbor-tristis is frequently referred to as Night Jasmine. Numerous ailments, including sciatica, rheumatism, internal worm infections, chronic fever, laxative, diaphoretic, and diuretic, are treated with the herb. The current study's goal was to use animal models to examine the antinociceptive effects of an ethanolic extract of Nyctanthes arbor-tristis leaves (EENA). Methods: A wide range of mouse models of acute pain, including the hot plate, tail immersion, acetic acid-induced writhing, formalin, and glutamate-induced tests, were used to measure the antinociceptive activity. The conventional medications were administered intraperitoneally as diclofenac sodium (10 mg/kg) and morphine sulphate (5 mg/kg). Oral dosages of 50, 100, and 200 mg/kg of EENA were given, whereas the control group was given deionised water (0.1 mL/mouse, P.O.). These techniques evaluate potential antinociceptive substances or plant extracts using traditional nociception models. Results: According to the current research, EENA strongly reflected the antinociceptive activity of all chemical and heat-induced pain models in mice. In contrast to thermal-induced nociception, 200 mg/kg showed a substantial (*p