The Impact of Acoustic Vibration and Circadian Disruption on the Secondary Metabolism of Ocimum sanctum-PDF

This study was designed to optimize the biosynthesis of medicinal phytochemicals in Ocimum sanctum through controlled abiotic stress. The effects of circadian disruption (2-hour light/1 hour dark and 24-hour light) and sound frequency exposure (300 Hz and 500 Hz) on the concentrations of rosmarinic acid, caffeic acid, luteolin, and apigenin were determined. Five groups of plants were subjected to stressors, followed by leaf harvesting and ethanol extraction. Phytochemical yields were quantified using Thin Layer Chromatography (TLC) and a custom digital imaging pipeline to calculate spot density. Results indicate that targeted abiotic stressors effectively upregulate secondary metabolites.