Effects of troxerutin and selenium supplementation in mitigating cypermethrin-induced behavioral impairments and oxidative stress in mice

The current research aimed to investigate the potential effectiveness of single and combined supplementationwith troxerutin (Trx) and selenium (Se) against cypermethrin (Cyp)-induced neurotoxicity and oxidative stress in mice.Thirty mice were randomly divided into five groups. The control group received 0.9% normal saline orally; the Cyp groupreceived Cyp (5 mg/kg) orally; the Cyp+Trx group received Trx (150 mg/kg) in combination with Cyp; the Cyp+Se groupreceived Se (25 ?g/kg) with Cyp; and the Cyp+Trx+Se group received Trx (150 mg/kg) and Se (25 ?g/kg) together with Cyp,all administered orally for 28 days. The individual and combined supplementation of Trx and Se significantly improvedmotor impairment, memory function, hepatic and renal health markers, and reduced anxiety levels (inside time/time incentar zone) in Cyp-exposed mice. Moreover, all supplementations reduced the Cyp-induced oxidative stress, but the SODactivity was increased in the serum and brain by the supplementation of Trx and Se, respectively. Liver and kidney SODactivities were improved with all the supplementations. Combined supplementation of Trx and Se enhanced brain acetylcholinesteraseactivity compared to the Cyp group. These findings suggest that both single and combined supplementationwith Trx and Se confer neuroprotection against pesticide-induced oxidative and behavioral alterations; however, brainacetylcholinesterase activity and renal health markers were improved only by the combined Trx and Se supplementation.