Exploration of the Improved Preparation Method of Rabbit Acellular Adipose Matrix

Tissue engineering requires biorepair materials with higher cost performance, and acellular adipose matrix has broad clinical application space. Therefore, we have improved and explored the preparation method of acellular adipose matrix of rabbit, evaluated its cytocompatibility, and verified the feasibility of its preparation method and its potential as a tissue repair material. The adipose tissue derived from rabbits was decellularized by an improved method. The appearance morphology and DAPI staining after preparation were observed, and the effects of the extracellular matrix of rabbit decellularized cells on the cytoskeleton morphology and migration ability of adipose-derived stem cells were studied. At the same time, in vivo animal transplantation experiments were carried out. The rabbit acellular adipose matrix in this experiment is gelatinous and milky white. DAPI staining leaves no residual cellular components. There are no abnormalities in the cytoskeleton or physiological activities of the cells. The prepared acellular adipose-derived extracellular matrix can be induced to form lipids in vivo. By applying the improved decellularization method, the rabbit acellular adipose matrix has no toxic effect on the cells can be successfully prepared.