https://passer.garmian.edu.krd/article_236457.html

Vibrio cholerae is a gram-negative, comma-shaped bacterium causing cholera, a severe diarrheal disease. This pathogen remains a major public health threat in areas with inadequate water and sanitation infrastructure, spreading through contaminated water and causing rapid dehydration. Genetic analysis using 16S rRNA sequencing and PCR-based detection of the ctxA gene confirmed the virulence potential of the isolate. Antimicrobial testing showed complete sensitivity (100%) to imipenem, azithromycin, ciprofloxacin, cefotaxime, and ceftriaxone, with doxycycline demonstrating 90.9% sensitivity. However, significant resistance emerged for amikacin (81.8%), cephalexin (63.6%), chloramphenicol (45.5%), and erythromycin (45.5%). Biofilm formation assessment revealed that virtually all isolates possessed this capability. This study elucidates the pathogenic determinants and antimicrobial resistance repertoire of Vibrio cholerae isolates, with phenotypic and genotypic characteristics confirmed through standardized testing protocols.