IL-21通过STAT3信号通路调控鱼类CD8+ T细胞免疫与抗菌防御

CD8⁺ T cells are indispensable effectors of adaptive immunity. While cytokine-mediated regulation of CD8⁺ T cell responses has been extensively characterized in mammals, the underlying mechanisms in fish remain largely unexplored. Here, we identified and characterized an interleukin (IL)-21 gene from Nile tilapia (Oreochromis niloticus). In vitro stimulation with IL-21 significantly upregulated the transcription and expression of key CD8⁺ T cell effector molecules, including granzyme B and interferon-gamma (IFN-γ) in splenic leukocytes, indicating a crucial role of IL-21 in modulating CD8⁺ T cell function in teleosts. Using a monoclonal antibody (mAb) against Nile tilapia IL-21, we further demonstrated that activated CD4-1⁺ T cells are the primary producers of this cytokine in fish. Intraperitoneal injection of recombinant IL-21 into Edwardsiella piscicida-infected Nile tilapia enhanced both proliferation and apoptosis of CD8⁺ T cells. Moreover, IL-21 elevated the expression of granzyme B, IFN-γ, and IL-2, and augmented CD8⁺ T cell cytotoxicity, thereby strengthening their effector functions. In contrast, blockade of IL-21 signaling with the mAb severely impaired CD8⁺ T cell responses. Mechanistically, Nile tilapia IL-21 strongly induced phosphorylation of signal transducer and activator of transcription 3 (STAT3), which was required for IL-21–mediated CD8⁺ T cell proliferation in vitro. Inhibition of STAT3 phosphorylation in vivo attenuated CD8⁺ T cell responses and antibacterial immunity, underscoring its functional importance. Collectively, our findings establish IL-21 as a pivotal regulator of CD8⁺ T cell immunity in fish and provide evolutionary insight into conserved cytokine pathways shaping adaptive immune responses.