The study of the acute toxicity and pharmacological activity of the Salvia splendens herb thick extract

Aim. To study the acute toxicity, anti-inflammatory and antioxidant activity of the Salvia splendens herb thick extract. Materials and methods. To study the acute toxicity, the Salvia splendens herb thick extract was administered to experimental animals in the dose of 5000 mg/kg. When assessing the anti-inflammatory activity, the anti-exudative effect was studied in a model of acute paw edema in rats caused by subplantar administration of 0.1 ml of 1 % carrageenan solution under the aponeurosis of the hind right limb. The effectiveness of the application was evaluated compared to animals of the control pathology group and reference drugs – diclofenac sodium in the dose of 8 mg/kg and quercetin in the dose of 50 mg/kg. The activity of oxidative processes was evaluated by the catalase activity and the content of TBA-reactive products. Results. The acute toxicity study has not revealed any cases of mortality in experimental animals when administered in the dose of 5000 mg/kg, indicating the absence of toxic effects of the extract studied in overdose. It has been found that the extract, according to K. K. Sidorov classification, belongs to the class of practically non-toxic substances (LD50 > 5000 mg/kg). When studying the anti-exudative activity, it has been determined that the extract under research has the anti-inflammatory effect and can be used for the further development of drugs aimed at reducing the inflammatory process in the body. However, the extracts studied do not have a sufficient level of the antioxidant activity and cannot actively suppress oxidative stress in the inflammatory process. Conclusions. It has been determined that the Salvia splendens herb thick extract belongs to the class of practically non-toxic substances. The anti-inflammatory effect of the extract studied is manifested by inhibition of exudative processes. The antioxidant activity assessment has shown its low effectiveness in suppressing oxidative stress in the inflammatory process