Exploring The Potential of HPTLC as a Method for Measuring Quercetin and Confirming Its Efficacy

Cinnamomum tamala has historically been utilized for the treatment of diabetes, metabolic diseases, and renal issues. This paper introduces a validated HPTLC technique for the precise measurement of quercetin in a 70:30 methanolic leaf extract. The optimal separation was achieved using a solvent mixture of toluene, ethyl acetate, and diethyl amine at a ratio of 8.0:2.5:0.5 (v/v/v) on silica gel plates, followed by a densitometric scan at 365 nm. The approach yielded a distinct quercetin peak at Rf 0.65, with limits of detection (LOD) and quantitation (LOQ) of 9.32 ng/band and 83.92 ng/spot, respectively. The extract contained 7.6% w/w quercetin. Validation in accordance with ICH standards demonstrated that the results were accurate and exact. This enhanced HPTLC technique is a rapid, sensitive, and reproducible approach for standardizing C. tamala extracts, ensuring dependable quality control.