Supplementary Material for Bao et al., 2025

Figure S1: Reporter gene activity of control (wol20X), negative mutants (Neg-Mut), two independent Low mutants, and two independent INXS mutantsFigure S2: PCR amplification confirming chromosomal junctions in INXS1Figure S3: PCR amplification confirming chromosomal junctions in INXS2Figure S4: Expression along chromosome 3R in mutant testisFigure S5: Expression along the X chromosome in mutant HT (head and thorax)Table S1: New mutations in the INXS1 and INXS2 mutantsTable S2: Single-cell marker genes for Drosophila testisVideo of dissected testes showing sperm motility of wild-type (wol20X) and INXS1 and INXS2 mutants