Quantitative Assessment of Clearing Protocol Efficacy for Multi-Dimensional Microscopy

Light scattering properties of biological tissue often limit imaging depth and resolution when acquiring images; optical tissue clearing significantly reduces light scattering, boosting the signal-to-noise ratio and providing crucial spatial information for deep imaging. Here, we present a user-friendly, open-source analytical pipeline for assessing tissue clearing efficacy, and illustrate our pipeline with a simple proof of concept. This pipeline provides a standard framework for the quantitative analysis of clearing protocols, allowing life science researchers to select an optimal protocol for their specific needs.Poster presented as part of the Crick BioImage Analysis Symposium 2025.Permission has been given by authors to upload to Crick Figshare. Copyright remains with the original authors.