Amplicon sequencing of phoD- and pqqC-harboring bacterial communities

The study site was located at Zhujiabu field experiment station, Jiande Forestry Farm, in western Zhejiang Province, China. Soil sample collection was carried out in October 2022. Within each sample plot, five soil cores (20 cm depth, 5 cm diameter) were collected randomly and sieved through a 2-mm sieve and mixed to form one composite sample as bulk soil per plot. The soil surrounding one of the main roots of the target individual was grubbed until a branch with a diameter of less than 2 mm was found, and the above procedure was repeated to collect three complete root branches.