STARR-seq in Upland Cotton for Identifying Cis-regulatory Elements**
Abstract
To obtain the raw STARR-seq data for identifying cis-regulatory elements, this study adopted the following experimental workflow: first, plasmid constructs containing 600-1500 bp test sequences were transfected into cotton protoplasts; then, fluorescence-activated cell sorting was used to isolate cell populations exhibiting GFP fluorescence (indicative of enhancer activity); and finally, high-throughput STARR-seq libraries were constructed using the SMART-seq2 method.
Fields of Study & Subjects
Research in Cotton Cultivation
Genomics and Chromatin Dynamics
Chromosomal and Genetic Variations
Publication details
- Journal / source
- China National GeneBank DataBase
- Publication date
- 1 January 2026
- Language
- EN
- Document type
- Dataset