Movie 1: 3D visualization of the adult human caudal superior hypogastric plexus (SHP)

A sample block of the adult human (male, 75 years) caudal superior hypogastric plexus (SHP) of an embalmed specimen processed for tissue clearing and viewed by light sheet fluorescence microscopy using 561 nm autofluorescence (no antibody staining).Neural tracts and blood vessels can be clearly observed using autofluorescence without additional staining. Some strong vascular autofluorescence is present, primarily due to residual blood cells from the fixation method. Neural tracts in distinct planes were segmented using syGlass and are shown in yellow, green, and blue. A blood vessel is segmented in red. The arrangement of these structures can clearly be seen in a transverse plane using the Imaris Ortho Slicer tool. In a coronal plane, regions of interconnecting fascicles, neuronal cell bodies embedded in the tracts and vascular branching are evident.Human tissue specimens were obtained from the organ donation program based at the Centre Hospitalier Universitaire de Nîmes (CHU de Nîmes), France and the Body Donor Program at the University of Melbourne, Australia. Ethical approval for the use of human tissue was granted from Human Research Ethics Committees at both institutions.