Testing of a biopreparation with iron oxide nanoparticles on flax seeds of varying prematurity

In the study, iron oxide Fe 3 O 4 nanoparticles synthesized using green tea extract and a solution of ferrous sulfate (FeSO 4 ∙ 7H 2 O) at a concentration of 0.1 mol/l were introduced in various doses into a finished liquid-phase biopreparation (LPB) at the maturation stage. The efficiency of the LPB-Fe line of biopreparations was studied in a laboratory experiment on flax seeds of the Nadezhda and Diplomat varieties of varying prematurity. The obtained results showed that the use of Fe 3 O 4 nanoparticles is an effective method for upgrading the original biopreparation. In all experimental variants, when testing the LPB-Fe biopreparations, an increase in germination energy and seed viability by 10–20% was noted, as well as an increase in the average length of seedlings by 4–5 cm and their average weight by 5–26 mg. For a more complete understanding of the effect of biopreparations on the plant organism, the correlation coefficients ( r ) between the microbiological and biochemical parameters of the obtained biopreparations and the average values for the variant of germination energy, viability and biometric data of flax seedlings were calculated. A moderate inverse relationship was found between the germination of flax seeds of the Nadezhda variety and the number of microorganisms using organic forms of nitrogen ( r = –0.64). A moderate direct relationship was also found between the activity of catalase and dehydrogenase enzymes and the average length of one seedling ( r = 0.56 and 0.66, respectively). Whereas when studying the seeds of the Diplomat variety, the average weight of one raw sprout strongly correlated with the number of microorganisms using mineral forms of nitrogen ( r = 0.85) and the total number of microorganisms using mineral and organic forms of nitrogen ( r = 0.91).