Uji Efektivitas Pemberian Salep Agathis Terhadap Penyembuhan Luka Insisi pada Tikus (Rattus Norvegicus)

Background: Wound healing is a complex biological process involving the phases of inflammation, proliferation, and tissue remodeling. Various topical agents have been developed to accelerate this process by reducing inflammation, preventing infection, and promoting tissue regeneration. Aims: This study aimed to evaluate the effectiveness of Agathis ointment in accelerating excisional wound healing in rats (Rattus norvegicus). Methods: A total of 18 male Sprague Dawley rats were divided into three treatment groups: a negative control group (no treatment), a positive control group (treated with commercial gentamicin ointment), and a treatment group (treated with Agathis ointment). Excisional wounds were created using a 5 mm punch biopsy tool, and treatments were applied twice daily for 10 days. Macroscopic observations were conducted by measuring wound area on days 3, 7, and 10, while microscopic observations included histological evaluation of inflammatory cell counts. Results: TThe results showed that Agathis ointment significantly (p>0.05) reduced wound area and inflammatory cell numbers, particularly on day 10, with outcomes comparable to or better than the commercial ointment group. This effectiveness is presumed to result from the synergistic effects of active compounds in Agathis ointment, such as acetylsalicylic acid, gentamicin, bioplacenton, lever-tran, and Peru balsam, which possess anti-inflammatory, antimicrobial, and tissue regeneration-promoting properties. Conclusion: In conclusion, Agathis ointment shows promising potential as an effective topical agent for accelerating wound healing in experimental animal models.