AVALIAÇÃO DO EFEITO DO BINÔMIO CONCENTRAÇÃO DE SOLVENTE X TEMPERATURA NA EXTRAÇÃO DE PIGMENTOS DA MICROALGA Chlorella vulgaris EM REGIME AUTOTRÓFICO

ABSTRACT Microalgae cultivation has gained prominence due to the great applicability of its biomass components, such as pigments, food industry, medicines and cosmetics. Given this scenario, this work aims to evaluate the influence of ethanol concentration and bath temperature on the extraction of pigments from Chlorella vulgaris microalgae, through the application of the Central Rotational Composite Design (DCCR). For the production of biomass used in the extraction, three bubble column photobioreactors, artificial lighting and BBM culture medium were used. All cultivation was evaluated according to a growth curve and kinetic parameters. At the end of the test, all the biomass microalgae used was used in the liquid medium by centrifugation, and the extractive process was performed afterwards based on the bath temperature conditions and the ethanol concentration used by the submitted DCCR. The kinetic parameters found were: PXmax= 0,003 ± 0,000 g/L.h, μmax= 0,010 h-1 ± 0,003 e tg = 74,37 ± 19,86h. An analysis of the statistical results of the pigment extraction process showed that the ethanol concentration promotes applied linear effects, 95% confidence, in the concentration of chlorophyll a, chlorophyll and carotenoids. The temperature, on the other hand, shows how significant it is in detecting chlorophyll a. These effects were graphically represented by the response surface methodology and it was possible to visually determine as ranges of the parameters that improve pigment extraction. As displayed ranges demonstrated at high temperatures (60 °C and 100 °C), for chlorophyll a cases, and high ethanol temperatures (80% and 100%), they are able to increase the yield of chlorophylls and carotenoids.