Micropropagation of genetically stable Jasminum leptophyllum - a narrow endemic shrub for conservation purpose

Endangered endemic plants face critical survival challenges, often hindered by reproductive barriers such as deep seed dormancy.Current study provides a meaningful contribution to conservation -by optimization of micropropagation protocol for Jasminum leptophyllum Rafiq, a critically endangered species-overcoming its major propagation constraint.A dual pre-treatment of sulfuric acid scarification followed by culture on a simplified, sucrose-free half-strength MS medium increased germination from 26.9% to 38.5%.Shoot multiplication was achieved with 0.4 mg/L BAP for multiple bud induction, while a previously unreported response was observed using 3 mg/L BAP + 4 mg/L Kinetin combination significantly enhanced shoot elongation.Rooting was most efficient with 1.6 mg/L NAA or IAA, producing robust roots within 16 days.Crucially, genetic fidelity of regenerated plants was rigorously validated using RAPD and SCoT markers, confirming true-to-type propagation and ensuring preservation of the species genetic integrity.This robust verification underlines the protocol's reliability for ex situ conservation and potential reintroduction programs.By integrating an effective regeneration system with comprehensive genetic fidelity assessment, this study not only safeguards J. leptophyllum but also establishes a model framework for conserving other rare, recalcitrant taxa.This work represents a significant advancement in plant conservation biotechnology, demonstrating that high-efficiency micropropagation can be combined with molecular verification to secure the future of endangered plants.