Establishment of Rapid Detection Method for Monkeypox Virus in Captive Primates

In this study, we focus on the zoo scenarios where frequent human-animal contact occurs. we aim to establish an efficient, accurate, convenient, and animal-friendly detection method for monkeypox virus. By adopting Colloidal Gold Immunochromatography (CGIA) combined with non-invasive sampling strategies, effective monitoring of monkeypox virus was achieved. The study prepared a colloidal gold immunochromatographic test strip and comprehensively validated its performance. The results showed that the test strip exhibited excellent specificity, high sensitivity (0.5ng/ml), good intra-batch and interbatch repeatability, strong stability, and was unaffected by faecal sample matrices. The test strip can rapidly complete detection within 10-15 minutes. This research provides a powerful support for reducing the risk of zoonotic diseases in captive primates, safeguarding the health of relevant staff and the public, and strengthening infectious disease prevention and control