FORMULATION AND EVALUATION OF TOPICAL NANOEMULGEL FOR DIABETIC WOUND HEALING BY USING TRIGONELLA FOENUM GRAECUM AND AEGLE MARMELOS LEAVES EXTRACT

Aim: Formulation and Evaluation of Topical Nanoemulgel for Diabetic Wound Healing by using Trigonella Foenum Graecum and Aegle Marmelos Leaves extract.Methods: O/W nanoemulsions were prepared by the ultrasonication method.F1 to F7 different formulations of combination of Trigonella Foenum Graecum [TFG] and Aegle Marmelos Leaves[AML] extract loaded nanoemulsions were prepared successfully and evaluated.From evaluation study best nanoemulsion formulation was selected and further it was incorporated into 1% and 1.5% Carbopol to create a gel that would make applying medication topically more convenient.Results and Discussion: The nanoemulsion area was determined by using pseudoternary phase diagrams.TFG seed oil choosen as oil phase, Tween 80 and Propylene glycol used as surfactant and cosurfactant respectively.The nanoemulsion formulations were evaluated for physical appearance, particle size, zeta potential, rheology study, In-vitro permeation study.From this evaluation F3 formulation was selected for incorporate into the gel base to form Nanoemulgel.The nanoemulgel formulations were evaluated for rheological study, drug content determination, In-vitro permeation study, Anti-microbial activity; In-vitro wound healing activity.Anti-microbial activity of prepared nanoemulgel was compared with conventional gel.The wound healing capabilities of nanoemulgel was assayed by performing In-vitro cell migration studies on L929 cells and compared with standard drug cipladine.The % of cells migrated towards the wound of nanoemulgel and cipladine were found to be 37% and 81% in 48 hrs.