PHYTOCHEMICAL EVALUATION, IN-VITRO ANTIOXIDANT POTENTIAL AND IN-VITRO ANTIUROLITHIATIC ACTIVITY OF NIGELLA SATIVA SEEDS

Objective: The current line of investigation was focused at perusing the in-vitro anti-oxidant potential and anti -urolithiatic activities of aqueous extract of Nigella sativa seeds using various methods Materials and Methods: The dried seed powder was subjected to maceration method to result crude aqueous extract.Phytoconstituents present in aqueous extract was examined by performing preliminary phytochemical screening.Total Phenolic Content (TPC), Total Flavonoid Content (TFC), Total alkalodal content (TAC) and Antioxidant potential for crude extracts were studied by DPPH, FRAP and Total anti-oxidant assay method methods.Anti-urolithiatic activity was screened using nucleation and aggregation assays in-vitro.