ND5-ND6 of mitochondrial dna as a molecular marker for differentiations of two repair and uterine nelma herds "Kubenskaya" and "Obskaya"

The purpose of the work is to evaluate the possibility of using the ND5/ND6 mtDNA site as a polymorphic marker for nelma differentiation using the example of two repair and brood herds of nelma "Kubenskaya" and "Obskaya" of the Forvat fish farm in the Leningrad region. Materials and Methods . Nelma haplotyping by PCR-RDF based on polymorphism of restriction sites of endonuclease enzymes and determination of the nucleotide sequence by Sanger sequencing of the ND5/ND6 mtDNA region. Results . The features of the ND5/ND6 nucleotide sequence presented in the work in representatives of the genus Stenodus had not previously been considered as an informative molecular genetic marker. It was found that the nelma from the repair and broodstock formed from the producers of Lake Kubensky demonstrates genetic differences from the repair and broodstock of the nelma from the Ob River. The analyzed mtDNA region can be used as a molecular genetic marker when carrying out nelma haplotyping at fish hatcheries in samples of a significant volume to control measures to restore geographically remote natural populations of nelma.