Exploratory Research on Standardization of Key Parameters in In Vitro and In Vivo Models of Ischemic Stroke

Standardizing stroke models is crucial for experimental reproducibility.This study optimized key parameters in common models.In HT22 cells, oxidative stress (H₂O₂), ischemia-reperfusion (OGD/R), and chemical hypoxia (CoCl₂) models were established, defining optimal injury conditions.In C57BL/6J mice, the filamentinduced tMCAO model was employed to compare effects of ischemia durations (45, 60, 90 min).Results demonstrated that all cellular injuries exhibited concentration/time-dependent patterns.In mice, prolonged ischemia exacerbated neurological deficits and infarct volume.A 60-minute occlusion achieved an optimal balance between stable infarction and survival.This study establishes key parameters for these models, emphasizing the necessity of precise induction conditions to ensure reproducibility.The findings provide a standardized foundation for mechanistic and therapeutic research.