Evaluation of cytotoxicity and acidity of developed polymer membranes for intestinal anastomoses

Research Objective: In vitro experiment, evaluate the cytotoxicity and acidity of the water extract of new samples of polymer surgical membranes developed by the authors, designed to prevent intestinal anastomosis failure. Materials and methods. The materials of this study were new samples of multicomponent polymer membranes developed on the basis of KSMU o (5 groups differing in the addition of various antibacterial contrast agents) and an implantable biopolymer ElastoPOB membrane (BIOMIR Service JSC, Russia). The acidity of the aqueous extract (pH) of the membranes was evaluated in six study groups. The effect of membranes on the proliferative activity of fibroblasts was also evaluated using an MTT test. The data obtained during the study were statistically processed using descriptive and variational statistics (Me [25; 75]). The significance of the difference in pH values was determined using the Kruskall-Walis test in (p ≤ 0.05), in the case of the MTT test, analysis of variance was used. Results. A comparative assessment of the change in the pH level of the water extract revealed statistically significant differences in experimental groups No. 3, 4, 5 with the values of group No. 6 (p ≤ 0.05). Statistically significant differences were found between the control group (distilled water) and groups No. 1 (p = 0.000032) and No. 2 (p = 0.000029). Samples of these groups affect the proliferative activity of fibroblasts, inhibiting it by 13.75% and 16.25%, respectively. At the same time, no statistically significant effects on the proliferative activity of fibroblasts were found when assessing the cytotactic effect of samples of groups No. 3-6. Conclusion. When conducting an MTT test, samples containing Yogexol, Levofloxacin and Metronidazole do not affect the proliferative activity of fibroblasts, which allows us to recommend for further comprehensive testing. At the same time, the pH of samples of groups No. 3-5 also have lower values compared to groups No. 1-2, 6.