DETECTION OF CARVACROL COMPOUND IN THREE TYPES OF OREGANO OILS (IRANIAN, AMERICAN AND FRENCH) AND EVALUATION OF ITS EFFECTIVENESS AGAINST CERTAIN PATHOGENIC BACTERIA

This study was conducted to determine the phytochemical content of three types of Origanumvulgareoil, namely the Iranian, American and French types, in addition to extracting and purifying the carvacrol compound and evaluating its biological activity against certain pathogenic and biofilm-producing bacteria.The results of the chemical analysis of the compounds identified in the oil of the Iranian type of Origanumvulgare using chromatographymass spectrometry showed the presence of only three compounds: Durene at 38.05% at retention time R. Time was 4.898, Carvacrol at 60.90% at a retention time (R-Time) of 9.620, and the third compound was 1-Azabicyclo, which was the least abundant compound at 1.05% at a time of 14.281.The results of the analysis of oregano oil in the American variety revealed the presence of eight compounds, with the fifth compound, 2-Ethyl-1-methoxy-4-methylbenzene, having the highest percentage at 75.26% at a R-Time of 9.905, while the compound Carbonic acid, 6-chlorohexyl 2,2,2-trichloroethyl ester was the least abundant compound, with a percentage of 0.06% at a R-Time of 14.356.The results of the analysis of oregano oil in the French variety showed the presence of seven compounds, with 3,6-Dimethylbenzene-1,2-diamine, N-methyl having the highest percentage of occurrence at 75.24% and a R-Time of 9.906.The results also demonstrated the effectiveness of carvacrol by showing high inhibitory activity against Staphylococcus aureus and Escherichia coli.This compound also inhibited biofilm formation in these bacteria.The study also showed that the minimum inhibitory concentration (MIC) of the compound was 62.5 μg/ml for S. aureus and 125 μg/ml for E. coli.