The Effect of EPS of Biofilm on Expression of Bax and Bcl-2 Genes in Human Cervical Carcinoma Cell Line (HeLa)

Background: The third most common malignancy in women is cervical carcinoma (CC). worldwide and remains a major reason of cancer-related losses. between females. Even though the availability of conventional therapies, their efficacy is often limited, which has encouraged the search for novel agents that selectively target cancer cells. Recent studies suggest that bacterial biofilms may possess anticancer activity. Objective: Establish exopolysaccharides (EPS) as promising candidates for natural, cost-effective, and biologically compatible cancer therapies. Methods: In this study, the cytotoxic influence of extracellular polymeric substances (EPS) derived from Pseudomonas aeruginosa biofilms was assessed on HeLa cervical cancer cells via the methyl thiazolyl tetrazolium (MTT) test to calculate the IC50, or half-maximal inhibitory concentration. Following EPS treatment, apoptotic markers were analyzed by real-time PCR. Results: EPS exposure led to a significant, dose-dependent inhibition of HeLa cell proliferation matched to Vero cells (p