SYNTHESIS AND CHARACTERIZATIONOFCINNAMALDEHYDE 4-HYDROXY METHYLPHENYLHYDRAZONE DERIVED FROM AN ISOLATEDHYDRAZINEOF Agaricus bisporus: APPLICATIONINTHESPECTROPHOTOMETRIC DETERMINATIONOFCOBALT(II)IN BIOLOGICAL AND ENVIRONMENTALSAMPLES

Hydrazones, known for their versatility in analytical chemistry, continue to draw attention for their abilityto form stable and selective complexes with transition metals. Researchers have evaluated the possibleanalytical uses of hydrazones and their derivatives. One significant class of widely recognised analytical reagents are hydrazones.2–7 Keeping in view the analytical potential of hydrazones, the formationandcharacterisation of cinnamaldehyde 4-hydroxy methyl phenyl hydrazone (CHMPH), its biological andanalytical applications, as well as the extraction of hydrazine from brown mushrooms (Agaricus bisporus), were studied. Based on the above derivative and direct spectrophotometric techniques to determinetheamount of cobalt (II) in vitamin B12, alloy samples, vehicle exhaust, tea leaves and biological sampleswere studied utilising CHMPH. Cobalt is vital for vitamin B12 and has a significant role in bacteria'snitrogen fixation. In medicine, Cobalt-60 is employed as an effective tracer in radioactive studies andanticancer therapy. It is a crucial component of vitamin B12 (cyanocobalamin), and cobalt is also vital tohuman nutrition. Deficiency of cobalt results in stunted growth, and in excess concentrations, it inhibitsenzyme activity and biosynthesis.8-9 Several analytical techniques have been reported in the literaturefor the determination of hydrazone derivatives; among them, spectrophotometric techniques are the most popular due to their ease of use and lower cost.10 The development of a method that does not relyonsolvent extraction is important. Micellar solutions increase the stability of metal complexes and serveaseffective media for the detection of metal chelates.