Multiplex PCR to Differentiate Monkeypox Virus Clades

Emerging infectious diseases 2026 Open access

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Authors: Christopher T. Williams, Alessandra Romero-Ramirez, Adeleye Adesola Semiu, Samuel Oluwafunmbi Ifabumuyi, Caitlin Greenland-Bews, Susan Gould, Dominic Wooding, Collette Allen, Anushri Somasundaran, Nicodemus Mkpuma, Dorcas A. Gado, Jolly Amoche Adole
+15 more Abdulakeem Eniola Amoo, Abisola Ajoke Adeyemi, Laure Bosquillon de Jarcy, Christine Goffinet, Jake Dunning, Malcolm G. Semple, The International Severe Acute Respiratory, Esto Bahizire, Afolabi Akinpelu, Thomas E. Fletcher, Ana Cubas-Atienzar, Cristina Leggio, Adeyinka Adedeji, Adesuyi Ayodeji Omoare, Thomas Edwards
DOI: 10.3201/eid3202.250686
Cited by: 2
References: 12

Abstract

We designed a multiplex quantitative PCR to differentiate monkeypox virus clades. For clinical samples collected in the United Kingdom and Nigeria, sensitivity was 78% (95% CI 67.67%-86.14%) and specificity 94% (95% CI 80.84%-99.30%); for samples with cycle thresholds

Fields of Study & Subjects

Poxvirus research and outbreaks Bacillus and Francisella bacterial research Viral Infections and Outbreaks Research

Publication details

Journal / source
Emerging infectious diseases
Publisher
Centers for Disease Control and Prevention (CDC)
Volume
32
Issue
2
Pages
250–254
Publication date
1 February 2026
Language
EN