AV1R: 'AV1' Video Encoding for Biological Microscopy Data

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Authors: Yuri Baramykov
Cited by: 0

Abstract

Converts legacy microscopy video formats (H.264/H.265, AVI/MJPEG, TIFF stacks) to the modern 'AV1' codec with minimal quality loss. Typical use cases include compressing large TIFF stacks from confocal microscopy and time-lapse experiments from hundreds of gigabytes to manageable sizes, re-encoding MP4 files exported from 'CellProfiler', 'ImageJ'/'Fiji', and microscope software with approximately 2x better compression at the same visual quality, and converting legacy AVI (MJPEG) and H.265 recordings to a single patent-free format suited for long-term archival. Automatically selects the best available backend: GPU hardware acceleration via 'Vulkan' 'VK_KHR_VIDEO_ENCODE_AV1' (tested on AMD RDNA4; bundled headers, builds with any 'Vulkan' SDK >= 1.3.275), with automatic fallback to CPU encoding through 'FFmpeg' and 'SVT-AV1'. Audio tracks are preserved automatically. Provides a simple R API for batch conversion of entire experiment folders.

Fields of Study & Subjects

Video Coding and Compression Technologies Advanced Data Compression Techniques Cell Image Analysis Techniques

Publication details

Publication date
5 March 2026
Language
EN
Document type
Dataset