Non-specific Clones Hamper the Reliability of Immunoglobulin Light Chain Gene Rearrangements in Next-Generation Sequencing-Based Measurable Residual Disease Monitoring in Pediatric B-Cell Acute Lymphoblastic Leukemia: A Single-Center Cohort Study

Annals of Laboratory Medicine 2026 Open access

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Authors: Haipin Chen, Jing Miao, Linyang Fan, Juan Liang, Jingying Zhang, Weiqun Xu, Fenying Zhao, Diying Shen, Heping Shen, Hua Song, Susan Wu, Yongmin Tang
+2 more Tao Sun, Xiaojun Xu
DOI: 10.3343/alm.2025.0486
Cited by: 2
References: 21

Abstract

Background: ) rearrangements are unreliable biomarkers for NGS-based MRD monitoring. Methods: This single-center prospective observational cohort study enrolled 506 pediatric patients newly diagnosed as having B-ALL between 2018 and 2022. NGS was performed on bone marrow samples collected at multiple time points, including the end of induction and consolidation. Results: clones at 12 months in patients in remission from 12.4% to 2.5%. Conclusions: rearrangements for NGS-based MRD monitoring in pediatric B-ALL. Filtering out these clones may enhance accuracy by reducing false positives.

Fields of Study & Subjects

Acute Lymphoblastic Leukemia research Chronic Myeloid Leukemia Treatments CAR-T cell therapy research

Publication details

Journal / source
Annals of Laboratory Medicine
Publisher
Annals of Laboratory Medicine
Volume
46
Issue
4
Pages
406–416
Publication date
12 February 2026
Language
EN