Flow-based imaging reveals dissociated monocyte adhesion and transmigration patterns between TNF-α and diabetes-induced vascular inflammation

Frontiers in Immunology 2026 Open access

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Authors: Dilvin Semo, Xiaohe Liang, Mariel Schwietzer, Weiqi Wang, Xiaokang Hu, Marc Dorenkamp, P Mohana Priya, Joshua Martin Manogaran, Franklin Christopher Vincent, Holger Reinecke, Rinesh Godfrey
DOI: 10.3389/fimmu.2026.1827607
Cited by: 1
References: 75

Abstract

monocyte adhesion, transendothelial migration (TEM), and abluminal residence on HUVEC monolayers activated with TNF-α or T2DM serum under controlled shear stress. Both stimuli induced comparable monocyte adhesion (~40 cells by 3 min), but TEM efficiency diverged markedly: TNF-α promoted robust TEM (~18% by 5 min) whereas T2DM conditions showed severely impaired TEM (~5%). Under T2DM conditions, monocytes exhibited prolonged abluminal retention (median 85 min vs. 25 min; p

Fields of Study & Subjects

Cell Adhesion Molecules Research Barrier Structure and Function Studies Angiogenesis and VEGF in Cancer

Publication details

Journal / source
Frontiers in Immunology
Publisher
Frontiers Media SA
Volume
17
Pages
1827607–1827607
Publication date
10 July 2026
Language
EN