Differential physiological responses and transcriptome co-expression networks of salt-tolerant and salt-sensitive foxtail millet (Setaria italica (L.)) under salt stress

Introduction: (L.)), a stress-resilient cereal, exhibits marked natural variation in salt tolerance, yet the regulatory mechanisms underlying this divergence remain unclear. Methods: Here, we integrated physiological assessments, time-course transcriptome profiling, and weighted gene co-expression network analysis (WGCNA) to dissect salt stress responses in a salt-tolerant accession (SDT80) and a salt-sensitive accession (SDS81). Key indicators of ion balance and oxidative damage were measured, and co-expression modules and hub genes associated with salt tolerance were identified. Results: ratio, and reduced membrane lipid peroxidation compared with SDS81. Transcriptomic analyses revealed dynamic and genotype-dependent expression patterns: SDT80 preferentially activated abiotic stress-related pathways, whereas SDS81 showed enrichment in processes linked to photosynthetic inhibition and cellular injury. WGCNA identified 23 co-expression modules, among which two key modules were strongly correlated with treatment duration, ion contents, and oxidative stress indices. Hub-gene analysis suggested that one module functions as a regulatory core integrating transcriptional control, calcium signaling, and metabolic adjustment, while the other is mainly involved in detoxification, energy metabolism, and cell wall remodeling. Discussion: Collectively, our integrative network analyses indicate that salt tolerance in foxtail millet arises from coordinated regulatory networks coupling ion homeostasis, stress signaling, and metabolic reprogramming rather than single-gene effects, providing candidate targets for improving salt tolerance in millet and other crops.