Remineralization Efficacy of Grape Seed Oil Paste on Demineralized Enamel after Bracket Debonding Compared to Casein Phosphopeptide-Amorphous Calcium Fluoride Phosphate Paste: An In Vitro Study

Aims: The present study aimed to evaluate the remineralization potential of grape seed (GS) oil incorporated into a prepared paste with different concentrations and compare it with casein phosphopeptide-amorphous calcium fluoride phosphate (CPP-ACPF) on enamel caries induced on debonded enamel surfaces. Materials and Methods: Forty-four sound permanent premolars were gathered for this study. After mounting, bracket bonding, and then debonding, the teeth were arbitrarily divided into four groups. The artificial enamel caries was created by immersing the samples in a demineralization solution. The enamel surface treated with: Group I (n=11), Control negative (artificial saliva), Group II (n=11), CPP-ACFP paste. Group III(n=11) 5% GS (paste1). Group IV(n=11) 10 % GS (paste2). 8 specimens were exposed to Surface Micro Hardness test (SMH), and 3 were exposed to Scanning Electron Microscope with Energy Dispersive X-ray Spectroscopy (SEM-EDX) after a period of 30 days of treatment. Results: The results of SMH exhibited a highly statistically significant difference in surface micro-hardness after treatment between the study groups, and the greatest mean value of surface microhardness was seen in 5 % GS (paste1). EDX results exhibited no statistically significant difference after treatment among the study groups. SEM observations showed that a protective layer was formed on the surface of the treated groups. Conclusion: The grape seed oil used in this study displayed a potential remineralization effect in artificial carious lesions on enamel