Membrane-Mediated Nanoassembly of Lysozyme–Tannic Acid for Crystallization-Suppressed Nobiletin Delivery: Enhanced Cellular Uptake and Mucus Penetration
Abstract
To enhance the bioavailability of hydrophobic nobiletin (NOB), this study constructed nanoparticles (LT-NOB) via self-assembly of lysozyme and tannic acid (TA). The multivalent weak interaction network between TA and lysozyme effectively encapsulated amorphous NOB, inhibiting crystallization. The optimized LT-NOB exhibited a size of 212 nm, high encapsulation efficiency (89.5%), and drug loading (47.25%). Cellular uptake was significantly improved, primarily through macropinocytosis, followed by lysosomal escape and endoplasmic reticulum targeting. In Caco-2 and co-culture models, LT-NOB enhanced mucosal permeation by 75% and 50%, respectively, compared to free NOB. This work elucidates a robust strategy for stabilizing amorphous drugs and promoting their intestinal absorption, providing a foundation for advanced nanodrug delivery.
Fields of Study & Subjects
Bioactive Compounds in Plants
Tea Polyphenols and Effects
Advanced Drug Delivery Systems
Publication details
- Journal / source
- Biomolecules
- Publisher
- MDPI AG
- Volume
- 16
- Issue
- 2
- Pages
- 242–242
- Publication date
- 3 February 2026
- Language
- EN